Lyophilization of microbial cultures: Principles, techniques, and applications in long-term preservation

DOI: https://doi.org/10.70593/978-93-49307-52-0_13

Authors

Yashdeep Srivastava
Department of Biotechnology, Invertis University, Bareilly, Uttar Pradesh, India
https://orcid.org/0000-0001-6218-2370
Keshawanand Tripathi
Department of Biotechnology, Invertis University, Bareilly, Uttar Pradesh, India
https://orcid.org/0000-0002-2106-5896
Narendra Kumar
School of Biotechnology and Bioengineering, Institute of Advanced Research, Gandhinagar, Gujarat, India
https://orcid.org/0000-0002-5578-3255

Synopsis

Freeze drying (lyophilization), also known as lyophilization, is a widely used method for preserving microbial cultures by removing water from the samples while maintaining the integrity of the microbial cells (Atlas, 2010). This technique is particularly valuable for long-term storage of microorganisms, as it allows for the preservation of cell viability and functionality over extended periods (Smith, 2014; Tripathi et al., 2018; Yadav et al., 2021). 

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Chapter 13

Volume

Biotechnology Lab Techniques: Culture Media, Microscopy, and Microbial Analysis

Pages

78-80

Published

13 April 2025
Copyright (c) 2025 Keshawanand Tripathi; Yashdeep Srivastava, Narendra Kumar

How to Cite

Srivastava, Y. ., Tripathi, K. ., & Kumar, N. . (2025). Lyophilization of microbial cultures: Principles, techniques, and applications in long-term preservation. In K. . Tripathi, Y. . Srivastava, & N. . Kumar (Eds.), Biotechnology Lab Techniques: Culture Media, Microscopy, and Microbial Analysis (pp. 78-80). Deep Science Publishing. https://doi.org/10.70593/978-93-49307-52-0_13
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