Biotechnology Lab Techniques: Culture Media, Microscopy, and Microbial Analysis

Keshawanand  Tripathi; Yashdeep  Srivastava; Narendra  Kumar; Sarvesh Kumar  Pandey; Shashank  Upadhyay; Santosh Kumar  Mishra

doi:10.70593/978-93-49307-52-0

Biotechnology Lab Techniques: Culture Media, Microscopy, and Microbial Analysis

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Authors

Keshawanand Tripathi (ed)

Department of Biotechnology, Invertis University, Bareilly, Uttar Pradesh, India

https://orcid.org/0000-0002-2106-5896

Yashdeep Srivastava (ed)

Department of Biotechnology, Invertis University, Bareilly, Uttar Pradesh, India

https://orcid.org/0000-0001-6218-2370

Narendra Kumar (ed)

School of Biotechnology and Bioengineering, Institute of Advanced Research, Gandhinagar, Gujarat, India

https://orcid.org/0000-0002-5578-3255

Keywords:

Sterilization, Microbiology, Plant tissue culture, Laboratory safety, Microbial isolation, Autoclave sterilization, Microscopy

Synopsis

Welcome to the "Biotechnology Lab Techniques: Culture Media, Microscopy, and Microbial Analysis”. This comprehensive manual is designed to be an essential companion for students, researchers, and professionals in the field of life sciences. Whether you are just starting your journey into laboratory practices or looking to deepen your understanding of advanced techniques, this handbook provides clear and practical guidance.
The world of life sciences is built upon a foundation of rigorous laboratory work, where precision and technique are paramount. This handbook begins with an introduction to basic laboratory practices, ensuring that readers develop a strong grasp of fundamental skills. From handling laboratory equipment to mastering techniques like smear preparation and staining of microorganisms, each chapter is structured to build upon the last, offering a progressive learning experience.
Central to this handbook are detailed sections on laboratory equipment and tools, essential for conducting experiments effectively. Whether you are operating a compound microscope, utilizing an autoclave for sterilization, or conducting experiments with UV-Vis spectrophotometers, this handbook provides comprehensive insights into their functions and applications.
Preparing media for cultivating microorganisms is a crucial skill covered extensively in this handbook. From nutrient broths to specialized agar types like McConkey and Chocolate agar, each recipe is meticulously detailed to ensure successful growth and isolation of pure microbial colonies. Techniques such as spread plating and streak plating are explained step-by-step, empowering researchers to isolate and study microbes with precision.
Beyond basic techniques, this handbook delves into advanced topics such as the impact of environmental factors like UV radiation and pH on microbial growth. Techniques for assessing cell viability and methods for evaluating antibacterial efficacy of natural products are also explored in detail, reflecting the handbook's commitment to practical relevance in contemporary research.
Additionally, this handbook encompasses techniques in molecular biology and biochemistry, from isolating nucleic acids and proteins to conducting gel electrophoresis and protein estimation assays. These techniques are pivotal for advancing research in genetics, biotechnology, and pharmaceutical sciences.
Furthermore, the handbook extends its scope to include botanical and environmental sciences, featuring methods for estimating chlorophyll content, investigating organogenesis in plants, and assessing biochemical oxygen demand in water samples. Each chapter is authored by experts in their respective fields, ensuring that the content is not only informative but also reliable and up-to-date with current scientific practices.
In conclusion, " Biotechnology Lab Techniques: Culture Media, Microscopy, and Microbial Analysis " is more than just a reference guide; it is a practical companion that equips readers with the knowledge and skills necessary to excel in their scientific endeavors. Whether used in educational settings or research laboratories, this handbook serves as an indispensable tool for navigating the complexities of life sciences.

Chapters

Fundamentals of laboratory practices: Principles, techniques, and safety protocols

Narendra Kumar, Keshawanand Tripathi, Yashdeep Srivastava

DOI: https://doi.org/10.70593/978-93-49307-52-0_1

Chapter 1
Essential laboratory equipment and tools: Functions, handling, and applications

Narendra Kumar, Keshawanand Tripathi, Yashdeep Srivastava, Sarvesh Kumar Pandey

DOI: https://doi.org/10.70593/978-93-49307-52-0_2

Chapter 2
Composition, optimization, and preparation of culture media for microbiological and biotechnological applications

Narendra Kumar, Keshawanand Tripathi, Yashdeep Srivastava

DOI: https://doi.org/10.70593/978-93-49307-52-0_3

Chapter 3
Techniques for isolation of pure microbial colonies: Principles, methods, and applications

Narendra Kumar, Keshawanand Tripathi, Yashdeep Srivastava

DOI: https://doi.org/10.70593/978-93-49307-52-0_4

Chapter 4
Standardized techniques for microbial smear preparation: Principles, staining protocols, and microscopic evaluation

Narendra Kumar, Keshawanand Tripathi, Yashdeep Srivastava

DOI: https://doi.org/10.70593/978-93-49307-52-0_5

Chapter 5
Staining techniques for microorganisms: Principles, methods, and diagnostic applications

Narendra Kumar, Keshawanand Tripathi, Yashdeep Srivastava

DOI: https://doi.org/10.70593/978-93-49307-52-0_6

Chapter 6
Effects of ultraviolet (UV) radiation on microbial growth: Mechanisms, responses, and applications

Narendra Kumar, Keshawanand Tripathi, Yashdeep Srivastava

DOI: https://doi.org/10.70593/978-93-49307-52-0_7

Chapter 7
Influence of pH on microbial growth: Mechanisms, adaptations, and industrial implications

Narendra Kumar, Keshawanand Tripathi, Shashank Upadhyay, Sarvesh Kumar Pandey, Yashdeep Srivastava

DOI: https://doi.org/10.70593/978-93-49307-52-0_8

Chapter 8
Trypan blue dye exclusion assay: Principles, protocols, and applications in cell viability assessment

Keshawanand Tripathi, Yashdeep Srivastava, Narendra Kumar

DOI: https://doi.org/10.70593/978-93-49307-52-0_9

Chapter 9
Evans blue dye exclusion assay: Mechanisms, protocols, and applications in cell viability analysis

Keshawanand Tripathi, Yashdeep Srivastava, Narendra Kumar

DOI: https://doi.org/10.70593/978-93-49307-52-0_10

Chapter 10
Paraffin block preparation and histological staining: Techniques for tissue structure analysis

Keshawanand Tripathi, Yashdeep Srivastava, Narendra Kumar

DOI: https://doi.org/10.70593/978-93-49307-52-0_11

Chapter 11
Paraffin preservation of microbial cultures: Techniques, mechanisms, and long-term storage applications

Keshawanand Tripathi, Yashdeep Srivastava, Narendra Kumar

DOI: https://doi.org/10.70593/978-93-49307-52-0_12

Chapter 12
Lyophilization of microbial cultures: Principles, techniques, and applications in long-term preservation

Yashdeep Srivastava, Keshawanand Tripathi, Narendra Kumar

DOI: https://doi.org/10.70593/978-93-49307-52-0_13

Chapter 13
Assessment of antibacterial efficacy of selected natural products: Methods, mechanisms, and applications

Keshawanand Tripathi, Yashdeep Srivastava, Narendra Kumar

DOI: https://doi.org/10.70593/978-93-49307-52-0_14

Chapter 14
Quantitative analysis of bacterial growth: Methods for measuring growth curves and kinetic parameters

Keshawanand Tripathi, Yashdeep Srivastava, Narendra Kumar

DOI: https://doi.org/10.70593/978-93-49307-52-0_15

Chapter 15
Enumeration of soil microbiota: Calculation of colony forming units (CFU) for microbial quantification

Keshawanand Tripathi, Yashdeep Srivastava, Narendra Kumar

DOI: https://doi.org/10.70593/978-93-49307-52-0_16

Chapter 16
Replica plating technique for identifying E. Coli auxotrophic mutants: Principles, methods, and application

Yashdeep Srivastava, Keshawanand Tripathi, Narendra Kumar

DOI: https://doi.org/10.70593/978-93-49307-52-0_17

Chapter 17
Measurement of water potential: Comparative analysis of three distinct methods in biological systems

Keshawanand Tripathi, Yashdeep Srivastava, Narendra Kumar

DOI: https://doi.org/10.70593/978-93-49307-52-0_18

Chapter 18
Experimental demonstration of transpiration pull: Mercury method for measuring xylem water transport

Keshawanand Tripathi, Yashdeep Srivastava, Narendra Kumar

DOI: https://doi.org/10.70593/978-93-49307-52-0_19

Chapter 19
Estimation of biochemical oxygen demand (BOD): Principles, methodology, and water quality assessment

Keshawanand Tripathi, Yashdeep Srivastava, Narendra Kumar

DOI: https://doi.org/10.70593/978-93-49307-52-0_20

Chapter 20
Quantitative estimation of dissolved oxygen (DO) in water samples: Principles, methods, and environmental significance

Keshawanand Tripathi, Yashdeep Srivastava, Narendra Kumar

DOI: https://doi.org/10.70593/978-93-49307-52-0_21

Chapter 21
Isolation of bacterial chromosomal DNA: principles, techniques, and analytical applications

Yashdeep Srivastava, Keshawanand Tripathi, Narendra Kumar

DOI: https://doi.org/10.70593/978-93-49307-52-0_22

Chapter 22
Extraction and purification of plasmid DNA from bacteria: Techniques and applications in molecular biology

Yashdeep Srivastava, Keshawanand Tripathi, Narendra Kumar

DOI: https://doi.org/10.70593/978-93-49307-52-0_23

Chapter 23
CTAB-Based extraction of total plant genomic DNA: Principles, protocols, and applications

Yashdeep Srivastava, Keshawanand Tripathi, Narendra Kumar

DOI: https://doi.org/10.70593/978-93-49307-52-0_24

Chapter 24
Trizol-based RNA extraction: Principles, protocol, and applications in molecular biology

Yashdeep Srivastava, Keshawanand Tripathi, Narendra Kumar

DOI: https://doi.org/10.70593/978-93-49307-52-0_25

Chapter 25
Synthesis of complementary DNA (C-DNA): Principles, methodology, and applications in gene expression studies

Yashdeep Srivastava, Keshawanand Tripathi, Narendra Kumar

DOI: https://doi.org/10.70593/978-93-49307-52-0_26

Chapter 26
Agarose gel electrophoresis of DNA: Principles, protocols, and applications in molecular analysis

Yashdeep Srivastava, Keshawanand Tripathi, Narendra Kumar

DOI: https://doi.org/10.70593/978-93-49307-52-0_27

Chapter 27
Isolation and purification of recombinant proteins: Principles, techniques, and biotechnological applications

Yashdeep Srivastava, Keshawanand Tripathi, Narendra Kumar

DOI: https://doi.org/10.70593/978-93-49307-52-0_28

Chapter 28
SDS-PAGE Analysis of proteins: Principles, methodology, and applications in proteomics

Yashdeep Srivastava, Keshawanand Tripathi, Narendra Kumar

DOI: https://doi.org/10.70593/978-93-49307-52-0_29

Chapter 29
Experimental validation of lambert-beer’s law: Principles, spectrophotometric analysis, and applications

Yashdeep Srivastava, Keshawanand Tripathi, Narendra Kumar, Sarvesh Kumar Pandey, Santosh Kumar Mishra

DOI: https://doi.org/10.70593/978-93-49307-52-0_30

Chapter 30
Quantitative protein estimation using the Bradford assay: Principles, protocols, and applications

Yashdeep Srivastava, Keshawanand Tripathi, Narendra Kumar

DOI: https://doi.org/10.70593/978-93-49307-52-0_31

Chapter 31
Biuret assay for protein estimation: Principles, methodology, and analytical applications

Keshawanand Tripathi, Yashdeep Srivastava, Santosh Kumar Mishra, Narendra Kumar

DOI: https://doi.org/10.70593/978-93-49307-52-0_32

Chapter 32
Lowry method for protein quantification: Principles, protocols, and applications in biochemical analysis

Keshawanand Tripathi, Yashdeep Srivastava, Santosh Kumar Mishra, Narendra Kumar

DOI: https://doi.org/10.70593/978-93-49307-52-0_33

Chapter 33
Spectrophotometric estimation of phytosterols: Principles, methodology, and analytical applications

Yashdeep Srivastava, Keshawanand Tripathi, Narendra Kumar

DOI: https://doi.org/10.70593/978-93-49307-52-0_34

Chapter 34
Quantification of leaf chlorophyll content: Spectrophotometric estimation and physiological implications

Yashdeep Srivastava, Keshawanand Tripathi, Narendra Kumar

DOI: https://doi.org/10.70593/978-93-49307-52-0_35

Chapter 35
Investigation of organogenesis in bacopa monnieri: Mechanisms, tissue culture techniques, and developmental insights

Yashdeep Srivastava, Keshawanand Tripathi, Narendra Kumar

DOI: https://doi.org/10.70593/978-93-49307-52-0_36

Chapter 36

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Tripathi, K. ., Srivastava, Y. ., & Kumar, N. . (Eds.). (2025). Biotechnology Lab Techniques: Culture Media, Microscopy, and Microbial Analysis. Deep Science Publishing. https://doi.org/10.70593/978-93-49307-52-0

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