Quantitative analysis of bacterial growth: Methods for measuring growth curves and kinetic parameters

To comprehensively investigate bacterial population dynamics, Viable cells must be inoculated into a sterile broth medium, and the culture must be carefully maintained at ideal pH, temperature, and gas composition. (Stanier et al., 1986; Atlas, 2010; Yadav et al., 2021). These controlled parameters facilitate swift cellular proliferation, enabling the monitoring of microbial growth progression through a population growth curve (Monod, 1949; Reddy et al., 2019; Tripathi et al., 2018). This curve is delineated by plotting the rise in cell numbers during the incubation period.