Biuret assay for protein estimation: Principles, methodology, and analytical applications

DOI: https://doi.org/10.70593/978-93-49307-52-0_32

Authors

Keshawanand Tripathi
Department of Biotechnology, Invertis University, Bareilly, Uttar Pradesh, India
https://orcid.org/0000-0002-2106-5896
Yashdeep Srivastava
Department of Biotechnology, Invertis University, Bareilly, Uttar Pradesh, India
https://orcid.org/0000-0001-6218-2370
Santosh Kumar Mishra
Department of Life Science, Sharda School of Bioscience and Technology, Sharda University, Greater Noida, U.P, India
Narendra Kumar
School of Biotechnology and Bioengineering, Institute of Advanced Research, Gandhinagar, Gujarat, India
https://orcid.org/0000-0002-5578-3255

Synopsis

The Biuret reagent, made of sodium potassium tartrate and alkaline CuSO4 solution, is used to detect compounds with adjacent peptide bonds, forming a distinctive violet complex (Voet, et al., 2018). The intensity of this color directly indicates protein concentration, measured at 540 nm using a green filter. The Biuret method is highly sensitive, detecting protein concentrations as low as 1-2 mg/mL (González et al., 2008; Berg et al.,2015).

Downloads

Chapter 32

Volume

Biotechnology Lab Techniques: Culture Media, Microscopy, and Microbial Analysis

Pages

160-161

Published

13 April 2025
Copyright (c) 2025 Keshawanand Tripathi; Yashdeep Srivastava, Narendra Kumar

How to Cite

Tripathi, K. ., Srivastava, Y. ., Mishra, S. K. ., & Kumar, N. . (2025). Biuret assay for protein estimation: Principles, methodology, and analytical applications. In K. . Tripathi, Y. . Srivastava, & N. . Kumar (Eds.), Biotechnology Lab Techniques: Culture Media, Microscopy, and Microbial Analysis (pp. 160-161). Deep Science Publishing. https://doi.org/10.70593/978-93-49307-52-0_32
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