Quantitative protein estimation using the Bradford assay: Principles, protocols, and applications

In biochemical study, the Bradford method is a frequently used method for measuring protein concentration. This test, named for the scientist Marion M. Bradford who first presented it in 1976, is based on the absorbance spectrum of Coomassie Brilliant Blue dye shifting when it binds to proteins in an acidic environment (Bradford, 1976; Sambrook, and Russell, 2001; Yadav et al., 2016). The blue hue produced rises in intensity with protein concentration; it can be quantified spectrophotometrically at 595 nm (Voet et al., 2018; Yadav et al., 2021). Laboratory protein measurement often uses this fast, sensitive, and comparatively easy technique (Srivastava et al., 2022). It is important to remember, although, that detergents, reducing agents, and some interfering chemicals might affect the Bradford assay, hence appropriate sample preparation and controls are necessary. All things considered, the Bradford method provides an invaluable instrument for precisely estimating the amounts of proteins in different biological materials (Kruger, 2009).Bottom of Form