Enumeration of soil microbiota: Calculation of colony forming units (CFU) for microbial quantification

Quantifying bacterial cells or determining their density holds paramount importance in various standard procedures and assays within the realm of biology (Tortora, et al.,2021). Understanding cell counts serves as a cornerstone for monitoring cell growth, evaluating resistance during transformation and selection processes, seeding cells for further experimentation, and preparing for cell-centric studies (Alexander, 1977; Tripathi et al., 2018). The accuracy and consistency of bacterial cell counts are pivotal for precise quantitative analysis in subsequent phases (Cappuccino and Sherman, 2014).